Features 

• Dissolve and go
• Exactly pre-weighed tablets in bottles
• Mix elution buffer with phosphate buffer to achieve desired imidazole concentration
• Ultrapure, low UV₂₈₀ absorbance imidazole
• Formulated from analytical grade chemicals

Product description

Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago’s IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. 

IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300 mM sodium chloride, 50 mM sodium phosphate buffer with pH 8.0 at 22°C. One tablet of elution buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium phosphate buffer with pH 8.0 at 22°C.

Applications

• Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography
• The phosphate buffer may be used for other types of affinity chromatography methods

Directions for use

Prepare the phosphate and the elution buffers separately. Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water up to the given volume for each tablet and stir until full dissolution. Mix the phosphate buffer and elution buffer to achieve desired imidazole concentration, follow the dilution series included in the product sheet.

Shipping and storage

IMAC phosphate and elution buffer tablets are shipped at room temperature. Store the tablets in a dry place at room temperature. Shelf life for IMAC phosphate buffer tablets is five years and shelf life for IMAC elution buffer tablets is three years.

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets

Composition:

Phosphate Buffer

300 mM Sodium Chloride  

50 mM Sodium Phosphate

Elution Buffer

300 mM Sodium Chloride

300 mM Imidazole

50 mM Sodium Phosphate

Volume:

Phosphate Buffer: 400 ml
Elution Buffer: 100 ml

pH 8.0 ± 0.05 at 22°C

Shelf life:

Phosphate Buffer: 5 years after production date
Elution Buffer: 3 years after production date

MSDS: 

MSDS IMAC Elution Buffer Tablets (Swedish)

MSDS IMAC Phosphate Buffer Tablets (Swedish)