Absolute Lectins

Phaseolus vulgaris lectin P (PHA-P), native isomer composition

Article NoProduct namePack SizePriceQuantityAdd to cart
Article No: 
05-0038-10mg
Phaseolus vulgaris lectin P (PHA-P), native isomer composition
Pack Size: 
10 mg
Price: 105 EUR
Article No: 
05-0038-5mg
Phaseolus vulgaris lectin P (PHA-P), native isomer composition
Pack Size: 
5 mg
Price: 60 EUR

*** For pack size 1g, 10g or more, please contact us to inquire about pricing


Phaseolus vulgaris lectin P (PHA-P)

 

Features 

 

  • Ultrapure quality
  • Agglutinates leucocytes and erythrocytes
  • Mixture of L and E subunits
  • Sugar specificity: complex oligosaccharides
  • Lyophilized powder

 

 

 

 

Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits (1) incompositions L1E3, L2E2, L3E1, E4.

Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides (1). PHA-P is not blood group specific (2)and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.

Medicago’s PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band. PHA-P is available in vials or plastic bottlescontaining 1 g, 10 mg or 5 mg of lyophilized powder and theproduct is to be used for laboratory work only.

 

Applications

  • Lateral flow erythrocyte separation

 

Typical subunit composition
Total L subunit composition: 60-65%
Total E subunit composition: 35-40%

L4 25-35%
L3E1 20-30%
L2E2 10-20%
L1E3 17-27%
E4 1-11%

 

Directions for use

The lectin may be reconstituted in a suitable buffer for yourapplication.

 

Shipping and storage

The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.

 

References

1) Rigas, D.A. and Osgood, E.E. J. Biol. Chem. 212, 607, (1955)
(2) Kornfeld, R. and Kornfeld, S. J. Biol. Chem. 245, 2536, (1970)

MSDS: