SmartBuffers

PBS-Tween tablets - Phosphate Buffered Saline with Tween™ 20 pH 7.4

Article NoProduct namePack SizeSolution VolPriceQuantityBuy
Article No: 
09-8902-12
PBS-T pH 7.4 (3x12)
Pack Size: 
3x12 tablets
Solution Vol: 
500 ml/tablet
Price: 80 EUR
Article No: 
09-9410-10
PBS-T pH 7.4 (3x10)
Pack Size: 
3x10 tablets
Solution Vol: 
1000 ml/tablet
Price: 106 EUR
Article No: 
09-9410-10
PBS-T pH 7.4
Pack Size: 
10 tablets
Solution Vol: 
1000 ml/tablet
Price: 47 EUR
Article No: 
09-8902-12
PBS-T pH 7.4
Pack Size: 
12 tablets
Solution Vol: 
500 ml/tablet
Price: 36 EUR
Article No: 
09-8903-100
PBS-T pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
100 ml/tablet
Price: 38 EUR
Article No: 
09-8902-100
PBS-T pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
500 ml/tablet
Price: 149 EUR
Article No: 
09-9410-100
PBS-T pH 7.4
Pack Size: 
100 tablets
Solution Vol: 
1000 ml/tablet
Price: 237 EUR
PBS-T

Features

  • Formulated from analytical grade reagents
  • PBS with non-ionic detergent
  • Specifically developed for immunoassays
  • Exactly pre-weighed tablets
  • Dissolve and go for greater convenience

 

 


Product description

PBS-T buffer contains the non-ionic detergent Tween TM 20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. Decreasing the non-specific binding and staining makes ELISA results and blots easier to interpret.

Medicago’s PBS-T buffer is supplied as exactly pre-weighed tablets in bottles and in convenient blister packs. Final buffer volumes range from 100 ml to 1000 ml. One tablet dissolved in deionized water yields a 0.14 M sodium chloride 0.0027 M potassium chloride, 0.05 % TweenTM 20, 0.01 M phosphate buffer with pH 7.4 at 25°C.

 

Applications

  • Wash buffer in immunolabelling techniques, such as ELISA and Western blotting
  • Blocking buffer for plate based assays
  • Protein-plate coating

 

Directions for use

Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 50 ml to 300 ml of deionized water and stir the solution for a few minutes. Adjust up to the given final buffer volume, stir until full dissolution and the buffer is ready to use.

 

Shipping and storage

PBS-T buffer is shipped at room temperature. Store the product in a dry place at room temperature. Shelf life is three years after production date.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed tablets

Composition: 0.14 M NaCl, 0.0027 M KCl, 0.05% TweenTM 20, 0.010 M PO43-

Volume: 100 ml, 500 ml and 1000 ml

pH: 7.4 ± 0.05 at 25°C