Immuno Reagents

EC-Blue Enhanced™ Substrate (TMB)

Article NoProduct namePack SizePriceQuantityBuy
Article No: 
EC-Blue Enhanced, 100 ml
Pack Size: 
100 ml
Price: 32 EUR
Article No: 
EC-Blue Enhanced, 1000 ml
Pack Size: 
1000 ml
Price: 217 EUR
Article No: 
EC-Blue Enhanced, 250 ml
Pack Size: 
250 ml
Price: 56 EUR
EC Blue Enhanced™ Substrate


  • Ready-to-use liquid substrate
  • Substrate for assays using horseradish peroxidase (HRP) conjugate
  • Low background
  • High sensitivity
  • Long stability






Product description

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

One bottle of EC Blue consists of a solution containing proprietary ready-mixed chromogen substrate reagents, pH 3.8. The solution has a straw-yellow colour at room temperature and a light greenish tint when stored at 4°C. The colour returns to normal when warmed overnight to room temperature.

For laboratory use only.



  • Recommended for ELISA procedures


Storage and stability

Store at 4°C to 8°C. Do not freeze and do not expose to direct sunlight. Prolonged exposure to light is not recommended. Store in an amber bottle. As some metal ions can oxidize TMB, allow only glass or plastic to come in contact with the substrate.

Never pipette directly from the bottle. Do not leave the cap off. EC-Blue Enhanced™ Reagent is stable for at least 24 months when stored at 4°C to 8°C.


Directions for use

No additional mixing reagents are required. The material is not dependent on room temperature.


Shipping and Storage

The product is stable for 24 months if stored at 4°c to 8°C.



Appearance: Straw yellow colour at room temperature. Slightly green tint when stored at +4°C. Colour returns to yellow when stored at room temperature.

Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.                     

Functional characteristics:  Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.

Storage: 4°C to 8°C