Features

• Pure recombinant Protein A
• Immobilized on a cross-linked agarose matrix
• Strong binding to Fc region of IgG           
• High capacity: > 20 mg IgG/ml

Product description 

Immunosorb A consists of highly purified recombinant Protein A which has been derived from the bacterium Staphylococcus aureus. The protein is cloned and expressed in E.coli  and immobilized on a cross-linked agarose matrix.

Protein A binds to specific classes of immunoglobulins and can preferably be used for purification of mouse and rabbit immunoglobulins. The protein binds to the Fc region of immunoglobulins by interacting with the heavy chain. It binds with high affinity to human IgG₁ and IgG₂ as well as mouse IgG_2a and IgG_2b. Protein A binds to human IgM, IgA and IgE as well as to mouse IgG₃ and IgG₁ but with moderate affinity. It does not react with human IgG₃ or IgD, nor with mouse IgM, IgA or IgE.

The material is non-cultural, non-infectious and is not pathogenic for animals or plants. It does not contain any animal or cell culture derived products or additions such as albumin or serum nor any toxic or harmful substances.

Medicago’s Immunosorb A is provided as a liquid solution with 20 % ethanol as preservative in pack sizes 100 ml, 200 ml and 5 ml.

For research use only.

Applications 

• Purification of human, mouse and rabbit immunoglobulins
• Immobilization and detection of immunoglobulins
• Affinity chromatography

Directions for use 

No additional mixing reagents are required. The material is not dependent on room temperature.

Shipping and storage

The product is shipped at room temperature and is stable for 12 months if stored at 2°c to 8°C.

Specifications 

Appearance: White liquid solution

Source: Cloned and expressed in E.coli

Purity: > 95%

Preservatives: 20% ethanol

Binding specificity: Protein A binds to specific classes of immunoglobulins